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Image Search Results
Journal: BioMed Research International
Article Title: Inhibition of P-Selectin and PSGL-1 Using Humanized Monoclonal Antibodies Increases the Sensitivity of Multiple Myeloma Cells to Bortezomib
doi: 10.1155/2015/417586
Figure Lengend Snippet: P-selectin and PSGL-1 regulate adhesion of MM cells to endothelial and stromal cells in vitro . Endothelial and stromal cells were treated with increasing concentrations of SelG1 antibody (2.5, 5, and 10 μ g/mL) followed by adhesion of H929 or MM1.s cells labeled with calcein-AM. MM cell adhesion was assessed as a signal of adherent calcein-AM-positive MM cells measured by fluorescent reader and normalized to untreated cells (a). Likewise, H929 or MM1.s cells labeled with calcein-AM were incubated with increasing concentrations of SelK2 antibody (2.5, 5, and 10 μ g/mL) and plated on untreated endothelial and stromal cells, and the cell adhesion was measured as above (b). Stromal (c) and endothelial cells (d) were treated with SelG1 (10 μ g/mL) for 1 hr followed by plating MM1.s and H929 labeled with calcein-AM, and the adhesion was assessed as a signal of adherent calcein-AM-positive MM cells measured by fluorescent reader and normalized to untreated cells. Likewise, MM1.s and H929 were treated with SelK2 (10 μ g/mL) for 1 hr and plated on stromal (e) or endothelial cells (f), and the cell adhesion was measured as above. Values were considered significant for ∗ p < 0.05.
Article Snippet: The humanized monoclonal
Techniques: In Vitro, Labeling, Incubation
Journal: BioMed Research International
Article Title: Inhibition of P-Selectin and PSGL-1 Using Humanized Monoclonal Antibodies Increases the Sensitivity of Multiple Myeloma Cells to Bortezomib
doi: 10.1155/2015/417586
Figure Lengend Snippet: P-selectin and PSGL-1 affect proliferation of MM cells cocultured with endothelial and stromal cells in vitro . The effect of SelG1 antibody (10 μ g/mL) used on endothelial and stromal cells and SelK2 (10 μ g/mL) used on H929 cells on MM cell proliferation, with or without bortezomib (5 nM) treatment, analyzed by flow cytometry and normalized to untreated cells. Values were considered significant for ∗ p < 0.05.
Article Snippet: The humanized monoclonal
Techniques: In Vitro, Flow Cytometry